The Stagecoach bus was carrying five passengers during the collision yesterday afternoon (Sunday) on the A713 at Waterside.
The driver and a passenger of a Land Rover Discovery were taken to Ayr hospital with serious injuries. The incident happened at 12.20pm on the road between Patna and Dalmellington.
The bus windscreen was shattered after hitting the tree and there was debris scattered on the road. A spokeswoman for Stagecoach West Scotland said: "A car was in collision with one of our vehicles around 12 noon yesterday on the A713 at Waterside, Ayrshire.
Thankfully, none of the five passengers on board our vehicle or our driver was seriously injured. Safety is our absolute priority and we are helping Police Scotland inquiries into.
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